Can you take 25 mg of sertraline in AM and then take 10 mg of amitriptyline 12 hrs later?

I hope so!

I take 60mg amitriptyline and 50mg sertraline at bedtime.

Hi, A-S7!
Only if the same doctor prescribed them to be taken together and is closely supervising you. This is your Interaction Checker report which shows a potential major interaction:
https://www.drugs.com/interactions-check.php?drug_list=168-0,2057-0
It may be that your doctor has determined that the benefits for you outweigh this risk. If you have concerns you can talk to your doctor with this information.