Target Canine Ovulation Kit

Company: BioMetallics

Rapid Canine Progesterone Kit For Use in Veterinary Clinics

Components: 6 or 12 monoclonal antibody treated test cups.

6 or 12 sample applicators.

1 bottle of wash solution.

1 bottle of substrate buffer A.

1 bottle of substrate buffer B.

1 substrate mixing bottle.

1 bottle enzyme conjugate.

A color chart.

Target Canine Ovulation Kit Indications

This kit will enable the user to determine the optimum time for fertilization.

Accurate timing is important for breeding success especially when the breeding is done with fresh chilled or frozen semen.

The main reason for unsuccessful breedings is improper timing.

Test Principles: The visible signs of heat and breeding time (such as vulval swelling, end of vaginal discharge, or flagging) are only an approximate indication of the time of ovulation. These signs can vary by more than a week.

Ovulation is triggered by the Luteinizing Hormone or LH which peaks two days before ovulation. Progesterone levels begin to rise at the same time as the LH surge. Ovulation occurs two days after the initial rise in progesterone. Predicting ovulation is accurately done by detecting the initial rise in progesterone.

After ovulation occurs, the ovacytes take 2-3 days to ripen, making the fertile period 5-6 days after the initial rise in progesterone.

With the TARGET CANINE OVULATION Timing kit, it is possible to detect the initial rise in progesterone. The progesterone level before ovulation is low (between 0 and 1.0 ng/mL). This corresponds to a bright blue test result. When the initial rise in progesterone occurs, the test result will be a light blue.

Test Procedure: Test Preparation (For Serum or Plasma): Allow all kit components and samples to come to room temperature. Collect the blood sample into an EDTA or heparin coated tube (purple top tube) or in a dry tube (red top tube). Immediately after sample collection, invert the tube several times to mix. Spin the blood down with a centrifuge or allow the blood to clot by staying at room temperature for 1/2 to 1 hr and pour the serum into a clean glass tube. If the sample is not run immediately, store the sample in the refrigerator. For long term storage the sample should be frozen. Label the sample with the name, sample day and cycle day. Do not use whole blood. Do not run test with cold kits.

Guideline for When to Test:

1. One serum sample should be run during the first five days of the proestrus cycle (characterized by the presence of blood and vulval swelling) in order to determine the baseline progesterone level. The baseline progesterone level varies between 0-1 ng/mL in individual dogs and gives a bright blue color result similar to the C1 color on the color chart.

2. Begin testing to determine the initial rise in progesterone about 11-13 days after observing the first day of vaginal bleeding and vulval swelling (the beginning of proestrus). If the first result is the baseline color, bright blue (C1), then the progesterone level is still very low.

3. Retest every two days until the test results are a light blue that is similar to the C2 color on the color chart. This first fading of the color from C1 to C2 represents the initial rise in the progesterone level; ovulation will occur 1-2 days later.

4. Retest every day until the test results are faint blue similar to the C3 color on the color chart. This means the egg is beginning to ripen and the fertile period begins 2-3 days later. After obtaining a C3 color, natural matings and inseminations with fresh semen should take place in 2 or 3 days and again in 2 days. If using frozen semen, inseminate in 2 or 3 days and every other day for a total of 3 times.

5. If a white result is obtained, mating or insemination should be done immediately.

Storage

Store the kit in the refrigerator when not in use. Do not freeze.

Always reseal the plastic bag after removing a test cup.

Caution(s): Timing during Step 2 (time of the enzyme) is important: one (1) minute.

Do not exchange test cups or reagents between different kits.

If you want to do a control, do steps 2-7, the result should be C1.

Discussion: Other Uses: The Target Canine Progesterone kit can also be used to accurately determine the right time for a Cesarean section. About 24 hours before normal whelping, progesterone levels drop back to low baseline levels (and the Target test result changes from white (C4) to bright blue (C1)). By daily testing just before the expected whelping date, the time for the C-section can be precisely determined and emergency surgery or premature puppies can be avoided. Progesterone levels can also be measured throughout pregnancy in order to confirm that the progesterone levels remain high (C4) and the bitch is maintaining a normal pregnancy.

Presentation: 6 and 12 test kits.

CPN: 14850000